S. Rita Hospital, Atripalda Atripalda, Campania, Italy
Award: Presidential Poster Award
Gaetano Iaquinto, MD1, Vera Rotondi Aufiero, MSc2, Nicola Giardullo, MD3, Fabiana Capuano, MD3, Giuseppe Mazzarella, MSc2 1S. Rita Hospital, Atripalda, Atripalda, Campania, Italy; 2Institute of Food Sciences-CNR, Avellino, Campania, Italy; 3San G. Moscati Hospital, Avellino, Campania, Italy
Introduction: IL-15 has been showed to have a central role in the pathogenesis of coeliac disease (CD) in the development of mucosal damage, as well as in the functional impairment of T regulatory (Treg) cells.
Therefore, such cytokine appears to be a very relevant target for therapeutic intervention in CD.
In this study, we investigated the effect of a neutralizing IL-15 antibody on the mucosal immune response in CD, by using organ culture system.
Methods: mRNA transcripts of IFN-γ and TGF-β, pro- and anti-inflammatory cytokines, respectively, were analysed by quantitative reverse transcriptase-PCR (qRT-PCR) in intestinal biopsies from active CD patients (ACD) (n =9) cultured for 24 hours in medium with or without anti-IL-15 antibody .
Results: In ex vivo organ cultures of intestinal mucosa of active CD patients, anti IL-15 antibody induced a significant increase of TGF-β mRNA expression (*p< 0.05) and a trend toward a reduction of IFN-γ (not statistically significative).
Discussion: Our results suggest that antibody-mediated blockade of IL-15 reverse the Th1 inflammatory responses and restore Treg suppressor capacity in CD. Therefore, anti-IL-15 treatment might be considered as a novel therapy to normalize intestinal lymphocyte function in CD patients.
Figure: Fold change (y axis) represents messenger RNA expression normalized to GAPDH. Statistical significance is marked by respective symbols. (*p<0.05).
Disclosures:
Gaetano Iaquinto indicated no relevant financial relationships.
Vera Rotondi Aufiero indicated no relevant financial relationships.
Nicola Giardullo indicated no relevant financial relationships.
Fabiana Capuano indicated no relevant financial relationships.
Giuseppe Mazzarella indicated no relevant financial relationships.
Gaetano Iaquinto, MD1, Vera Rotondi Aufiero, MSc2, Nicola Giardullo, MD3, Fabiana Capuano, MD3, Giuseppe Mazzarella, MSc2. P2640 - Neutralizing IL-15 Inhibits Pro-Inflammatory Cytokine Production and Promote Protective Immune Responses, ACG 2023 Annual Scientific Meeting Abstracts. Vancouver, BC, Canada: American College of Gastroenterology.
